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transfection positive control expressing gfp  (Addgene inc)


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    Addgene inc transfection positive control expressing gfp
    mAb B: effects of LC/HC promoter strength on titer, cell density, and viability <t>post-transfection</t> (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey's HSD; different letters indicate p < 0.05. (D) Transfection efficiency (% <t>GFP+)</t> of a control plasmid (pMAX-GFP) quantified by flow cytometry. (E) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.
    Transfection Positive Control Expressing Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfection+positive+control+expressing+gfp/pMax-E2F1+(Plasmid+%2316007)/pmc12827772-30-54-61
    Average 93 stars, based on 18 article reviews
    transfection positive control expressing gfp - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength"

    Article Title: High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2025.1747473

    mAb B: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey's HSD; different letters indicate p < 0.05. (D) Transfection efficiency (% GFP+) of a control plasmid (pMAX-GFP) quantified by flow cytometry. (E) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.
    Figure Legend Snippet: mAb B: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey's HSD; different letters indicate p < 0.05. (D) Transfection efficiency (% GFP+) of a control plasmid (pMAX-GFP) quantified by flow cytometry. (E) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Techniques Used: Transfection, Control, Plasmid Preparation, Flow Cytometry

    mAb C: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (6), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean = SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.
    Figure Legend Snippet: mAb C: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (6), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean = SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Techniques Used: Transfection

    mAb E: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least- squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% CIs. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.
    Figure Legend Snippet: mAb E: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least- squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% CIs. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Techniques Used: Transfection

    Related Articles

    Transfection:

    Article Title: High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength
    Article Snippet: Escherichia coli Mach1 T1R chemically competent cells (C869601, Thermo Fisher Scientific, Waltham, MA, United States) 48 well LB agar plates (prepared in-house and supplemented with 60 μg/mL ampicillin) 2× YT medium: 16 g tryptone, 10 g yeast extract, and 5 g NaCl per liter of distilled water; pH adjusted to 7.0 and sterilized by autoclaving (121 °C, 20 min) SOC medium: prepared according to standard formulations (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose) Ampicillin (60 μg/mL, final concentration in liquid culture) .. CHO-S cells (R80007, Life Technologies, Waltham, MA, United States) CD CHO medium (10743029, Gibco, Thermo Fisher Scientific, Waltham, MA, United States) L-Glutamine (25030081, Thermo Fisher Scientific, Waltham, MA, United States) Freestyle MAX Transfection Reagent (16447100, Thermo Fisher Scientific, Waltham, MA, United States) OptiPRO serum free medium (12309019, Thermo Fisher Scientific, Waltham, MA, United States) Transfection positive control expressing GFP (pMax-E2F1, 16007, Addgene, Watertown, MA, United States) Solution 18 (910-3018, ChemoMetec A/S, Allerød, Denmark) NC-Slide A8 (941-0002, ChemoMetec A/S, Allerød, Denmark) .. MicroAmp Fast 96-Well Reaction Plate (4346907, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, United States) SimpliAmp Thermal Cycler (A24811, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, United States) Filtered pipette tips (10 μL, 200 μL, and 1000 μL; Thermo Fisher Scientific, Waltham, MA, United States) 1.5 mL and 2.0 mL microcentrifuge tubes (Eppendorf SE, Hamburg, Germany) Benchtop microcentrifuge (Eppendorf SE, Hamburg, Germany) Eppendorf Centrifuge 5810 R (Eppendorf SE, Hamburg, Germany) Gel electrophoresis system (Thermo Fisher Scientific, Waltham, MA, United States) 125 mL Erlenmeyer flasks with vent caps (431143, Corning Inc., Corning, NY, United States) 96-half-deep-well microplate (CR1496c, Enzyscreen B.V., Haarlem, The Netherlands) Autoclaved low-evaporation sandwich Duetz covers (CR1296a, Enzyscreen B.V., Heemstede, The Netherlands) Octet RED96 System (Sartorius, Göttingen, Germany) Protein A Biosensors (18-5010, Sartorius, Göttingen, Germany) Black 96-Well Microplates, Non-Binding Surface (3694, Corning Inc., Corning, NY, United States) Shaking incubator (37 °C, 350 rpm) Eppendorf New Brunswick S41i CO 2 incubator for mammalian cell culture (37 °C, 5% CO 2 , 70% humidity, 325 rpm, 2.5 cm orbital diameter) Amersham Imager 600 (AI600, Cytiva, Marlborough, MA, United States) NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States) NucleoCounter NC-200 (ChemoMetec A/S, Allerød, Denmark) CytoFLEX flow cytometer (Beckman Coulter, United States) Sterile disposable inoculating loop (10 μL; Deltalab, Barcelona, Spain)

    Positive Control:

    Article Title: High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength
    Article Snippet: Escherichia coli Mach1 T1R chemically competent cells (C869601, Thermo Fisher Scientific, Waltham, MA, United States) 48 well LB agar plates (prepared in-house and supplemented with 60 μg/mL ampicillin) 2× YT medium: 16 g tryptone, 10 g yeast extract, and 5 g NaCl per liter of distilled water; pH adjusted to 7.0 and sterilized by autoclaving (121 °C, 20 min) SOC medium: prepared according to standard formulations (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose) Ampicillin (60 μg/mL, final concentration in liquid culture) .. CHO-S cells (R80007, Life Technologies, Waltham, MA, United States) CD CHO medium (10743029, Gibco, Thermo Fisher Scientific, Waltham, MA, United States) L-Glutamine (25030081, Thermo Fisher Scientific, Waltham, MA, United States) Freestyle MAX Transfection Reagent (16447100, Thermo Fisher Scientific, Waltham, MA, United States) OptiPRO serum free medium (12309019, Thermo Fisher Scientific, Waltham, MA, United States) Transfection positive control expressing GFP (pMax-E2F1, 16007, Addgene, Watertown, MA, United States) Solution 18 (910-3018, ChemoMetec A/S, Allerød, Denmark) NC-Slide A8 (941-0002, ChemoMetec A/S, Allerød, Denmark) .. MicroAmp Fast 96-Well Reaction Plate (4346907, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, United States) SimpliAmp Thermal Cycler (A24811, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, United States) Filtered pipette tips (10 μL, 200 μL, and 1000 μL; Thermo Fisher Scientific, Waltham, MA, United States) 1.5 mL and 2.0 mL microcentrifuge tubes (Eppendorf SE, Hamburg, Germany) Benchtop microcentrifuge (Eppendorf SE, Hamburg, Germany) Eppendorf Centrifuge 5810 R (Eppendorf SE, Hamburg, Germany) Gel electrophoresis system (Thermo Fisher Scientific, Waltham, MA, United States) 125 mL Erlenmeyer flasks with vent caps (431143, Corning Inc., Corning, NY, United States) 96-half-deep-well microplate (CR1496c, Enzyscreen B.V., Haarlem, The Netherlands) Autoclaved low-evaporation sandwich Duetz covers (CR1296a, Enzyscreen B.V., Heemstede, The Netherlands) Octet RED96 System (Sartorius, Göttingen, Germany) Protein A Biosensors (18-5010, Sartorius, Göttingen, Germany) Black 96-Well Microplates, Non-Binding Surface (3694, Corning Inc., Corning, NY, United States) Shaking incubator (37 °C, 350 rpm) Eppendorf New Brunswick S41i CO 2 incubator for mammalian cell culture (37 °C, 5% CO 2 , 70% humidity, 325 rpm, 2.5 cm orbital diameter) Amersham Imager 600 (AI600, Cytiva, Marlborough, MA, United States) NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States) NucleoCounter NC-200 (ChemoMetec A/S, Allerød, Denmark) CytoFLEX flow cytometer (Beckman Coulter, United States) Sterile disposable inoculating loop (10 μL; Deltalab, Barcelona, Spain)

    Expressing:

    Article Title: High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength
    Article Snippet: Escherichia coli Mach1 T1R chemically competent cells (C869601, Thermo Fisher Scientific, Waltham, MA, United States) 48 well LB agar plates (prepared in-house and supplemented with 60 μg/mL ampicillin) 2× YT medium: 16 g tryptone, 10 g yeast extract, and 5 g NaCl per liter of distilled water; pH adjusted to 7.0 and sterilized by autoclaving (121 °C, 20 min) SOC medium: prepared according to standard formulations (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose) Ampicillin (60 μg/mL, final concentration in liquid culture) .. CHO-S cells (R80007, Life Technologies, Waltham, MA, United States) CD CHO medium (10743029, Gibco, Thermo Fisher Scientific, Waltham, MA, United States) L-Glutamine (25030081, Thermo Fisher Scientific, Waltham, MA, United States) Freestyle MAX Transfection Reagent (16447100, Thermo Fisher Scientific, Waltham, MA, United States) OptiPRO serum free medium (12309019, Thermo Fisher Scientific, Waltham, MA, United States) Transfection positive control expressing GFP (pMax-E2F1, 16007, Addgene, Watertown, MA, United States) Solution 18 (910-3018, ChemoMetec A/S, Allerød, Denmark) NC-Slide A8 (941-0002, ChemoMetec A/S, Allerød, Denmark) .. MicroAmp Fast 96-Well Reaction Plate (4346907, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, United States) SimpliAmp Thermal Cycler (A24811, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, United States) Filtered pipette tips (10 μL, 200 μL, and 1000 μL; Thermo Fisher Scientific, Waltham, MA, United States) 1.5 mL and 2.0 mL microcentrifuge tubes (Eppendorf SE, Hamburg, Germany) Benchtop microcentrifuge (Eppendorf SE, Hamburg, Germany) Eppendorf Centrifuge 5810 R (Eppendorf SE, Hamburg, Germany) Gel electrophoresis system (Thermo Fisher Scientific, Waltham, MA, United States) 125 mL Erlenmeyer flasks with vent caps (431143, Corning Inc., Corning, NY, United States) 96-half-deep-well microplate (CR1496c, Enzyscreen B.V., Haarlem, The Netherlands) Autoclaved low-evaporation sandwich Duetz covers (CR1296a, Enzyscreen B.V., Heemstede, The Netherlands) Octet RED96 System (Sartorius, Göttingen, Germany) Protein A Biosensors (18-5010, Sartorius, Göttingen, Germany) Black 96-Well Microplates, Non-Binding Surface (3694, Corning Inc., Corning, NY, United States) Shaking incubator (37 °C, 350 rpm) Eppendorf New Brunswick S41i CO 2 incubator for mammalian cell culture (37 °C, 5% CO 2 , 70% humidity, 325 rpm, 2.5 cm orbital diameter) Amersham Imager 600 (AI600, Cytiva, Marlborough, MA, United States) NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States) NucleoCounter NC-200 (ChemoMetec A/S, Allerød, Denmark) CytoFLEX flow cytometer (Beckman Coulter, United States) Sterile disposable inoculating loop (10 μL; Deltalab, Barcelona, Spain)



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    transfection positive control expressing gfp  (Addgene inc)
    93
    Addgene inc transfection positive control expressing gfp
    mAb B: effects of LC/HC promoter strength on titer, cell density, and viability <t>post-transfection</t> (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey's HSD; different letters indicate p < 0.05. (D) Transfection efficiency (% <t>GFP+)</t> of a control plasmid (pMAX-GFP) quantified by flow cytometry. (E) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.
    Transfection Positive Control Expressing Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfection+positive+control+expressing+gfp/pMax-E2F1+(Plasmid+%2316007)/pmc12827772-30-54-61
    Average 93 stars, based on 1 article reviews
    transfection positive control expressing gfp - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    mAb B: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey's HSD; different letters indicate p < 0.05. (D) Transfection efficiency (% GFP+) of a control plasmid (pMAX-GFP) quantified by flow cytometry. (E) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength

    doi: 10.3389/fbioe.2025.1747473

    Figure Lengend Snippet: mAb B: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey's HSD; different letters indicate p < 0.05. (D) Transfection efficiency (% GFP+) of a control plasmid (pMAX-GFP) quantified by flow cytometry. (E) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Article Snippet: CHO-S cells (R80007, Life Technologies, Waltham, MA, United States) CD CHO medium (10743029, Gibco, Thermo Fisher Scientific, Waltham, MA, United States) L-Glutamine (25030081, Thermo Fisher Scientific, Waltham, MA, United States) Freestyle MAX Transfection Reagent (16447100, Thermo Fisher Scientific, Waltham, MA, United States) OptiPRO serum free medium (12309019, Thermo Fisher Scientific, Waltham, MA, United States) Transfection positive control expressing GFP (pMax-E2F1, 16007, Addgene, Watertown, MA, United States) Solution 18 (910-3018, ChemoMetec A/S, Allerød, Denmark) NC-Slide A8 (941-0002, ChemoMetec A/S, Allerød, Denmark)

    Techniques: Transfection, Control, Plasmid Preparation, Flow Cytometry

    mAb C: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (6), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean = SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength

    doi: 10.3389/fbioe.2025.1747473

    Figure Lengend Snippet: mAb C: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (6), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean = SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least-squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% Cls. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Article Snippet: CHO-S cells (R80007, Life Technologies, Waltham, MA, United States) CD CHO medium (10743029, Gibco, Thermo Fisher Scientific, Waltham, MA, United States) L-Glutamine (25030081, Thermo Fisher Scientific, Waltham, MA, United States) Freestyle MAX Transfection Reagent (16447100, Thermo Fisher Scientific, Waltham, MA, United States) OptiPRO serum free medium (12309019, Thermo Fisher Scientific, Waltham, MA, United States) Transfection positive control expressing GFP (pMax-E2F1, 16007, Addgene, Watertown, MA, United States) Solution 18 (910-3018, ChemoMetec A/S, Allerød, Denmark) NC-Slide A8 (941-0002, ChemoMetec A/S, Allerød, Denmark)

    Techniques: Transfection

    mAb E: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least- squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% CIs. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: High-throughput optimization of antibody production in CHO cells by tuning heavy- and light-chain promoter strength

    doi: 10.3389/fbioe.2025.1747473

    Figure Lengend Snippet: mAb E: effects of LC/HC promoter strength on titer, cell density, and viability post-transfection (72 hpt), and model profiling. (A) Viability (%), (B) viable cell density (VCD; cells/mL), and (C) titer (mg/L) for all LC/HC combinations. Promoter levels: 5, 40, and 100 RPU for both LC and HC. Bars show mean ± SD (n = 2). Statistical analysis was based on one-way ANOVA with Tukey’s HSD; different letters indicate p < 0.05. (D) JMP profiler for least- squares models of titer, VCD, and viability versus LC/HC promoter strength. Left: predicted means with 95% CIs. Right: composite desirability (0-1) balancing high titer with acceptable VCD and viability. Red dashed lines mark targets/constraints.

    Article Snippet: CHO-S cells (R80007, Life Technologies, Waltham, MA, United States) CD CHO medium (10743029, Gibco, Thermo Fisher Scientific, Waltham, MA, United States) L-Glutamine (25030081, Thermo Fisher Scientific, Waltham, MA, United States) Freestyle MAX Transfection Reagent (16447100, Thermo Fisher Scientific, Waltham, MA, United States) OptiPRO serum free medium (12309019, Thermo Fisher Scientific, Waltham, MA, United States) Transfection positive control expressing GFP (pMax-E2F1, 16007, Addgene, Watertown, MA, United States) Solution 18 (910-3018, ChemoMetec A/S, Allerød, Denmark) NC-Slide A8 (941-0002, ChemoMetec A/S, Allerød, Denmark)

    Techniques: Transfection